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KMID : 0359320200600010001
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Korean Journal of Veterinary Research
2020 Volume.60 No. 1 p.1 ~ p.7
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Genotyping of the causative Leptospira in symptomatic dogs in Thailand
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Paungpin Weena
Chaiwattanarungruengpaisan Somjit
Mongkolphan Chalisa
Wiriyarat Witthawat
Thongdee Metawee
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Abstract
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We investigated the genotypes of Leptospira spp. detected in symptomatic dogs in Thailand. During April to December2012, 6 out of 41 client-owned dogs were diagnosed with leptospirosis based on polymerase chain reaction tests. All of the infecteddogs showed clinical symptoms related to leptospirosis. Direct genotyping of the causative agent of the canine leptospirosis wasconducted from the archival DNA samples extracted from urine or blood of those 6 infected dogs. Sequencing of the partial 16S rRNAand lipL32 genes from all samples identified Leptospira (L.) interrogans as the infecting species. Multilocus sequence typing testswere successful for 2 out of 6 samples. The sequence type (ST) was identified as ST50 for both samples where the profilecorresponded to L. interrogans species and Bataviae serogroup. The presence of this genotype of Leptospira has never been reportedin Thailand. Thus, our findings showed the existence of ST50 L. interrogans serogroup Bataviae and the ability to cause leptospirosisin dogs in Thailand.
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KEYWORD
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: Leptospira, genotype, leptospirosis, dog, MLST
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